What is the host animal and derivation of this Olokizumab antibody?

This product, Olokizumab (TAB-253), is a recombinant antibody. Its original host animal was a Rat. It has since been engineered as a Humanized antibody, meaning the antigen-binding regions from the rat antibody were grafted onto a human antibody framework. Specifically, its isotype is IgG4 - kappa. It is expressed in CHO cells and has a purity greater than 95%.

I'm looking for an antibody for neutralization assays. Is TAB-253 validated for this?

Yes, this antibody is well-suited for functional studies. The product data for TAB-253 explicitly lists Neutralization (Neut) as a reported application, in addition to others like WB, ELISA, FC, IP, and IF. It is designed to bind to human IL-6 and has been tested positive against the native human antigen, making it a reliable reagent for your neutralization experiments.

Anti-Human IL-6 Recombinant Antibody (Olokizumab)

CAT#: TAB-253

Recombinant monoclonal antibody to IL-6. Olokizumab is an immunomodulator. It binds to interleukin 6.

Tested Data Gene Expression

ELISA

Figure 1 Anti-Human IL6 Recombinant Antibody (TAB-253) in ELISA

ELISA analysis of TAB-253 was performed by coating with human IL6 protein (His tag). Then blocked with BSA and incubated with TAB-253. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-Human IL6 Recombinant Antibody (TAB-253) in WB

Western blot analysis of TAB-253 was performed by loading human IL6 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-253 and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.

Figure 3 Anti-Human IL6 Recombinant Antibody (TAB-253) in DB

Dot Blot analysis of TAB-253 was performed by coating with human IL6 protein (His tag).
TAB-253 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control

SDS-PAGE

Figure 4 Anti-Human IL-6 Recombinant Antibody (TAB-253) in SDS-PAGE

SDS-PAGE analysis of TAB-253 in non-reduced (Lane 1) and reduced (Lane 2) conditions.

SEC-HPLC

Figure 5 Anti-Human IL-6 Recombinant Antibody (TAB-253) in HPLC

HPLC analysis of TAB-253 shows the purity is>95%.

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Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72821/1350_G7_3_selected.jpg

Normal Tissue Figure 2 IHC staining of human duodenum

Immunohistochemical staining of human duodenum shows distinct cytoplasmic positivity in subsets of immune cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_8_2_selected.jpg

Normal Tissue Figure 3 Cerebral cortex

Neuronal cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_B_7_5.jpg

Normal Tissue Figure 4 Urinary bladder

Urothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_4_5.jpg

Normal Tissue Figure 5 Lymph node

Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23406/54890_A_9_8.jpg

RNA Expression Figure 6 RNA cell line category: Group enriched (BJ hTERT+, GAMG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136244-IL6

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Specifications

Immunogen
Recombinant full length protein (Human).

Host Species
Rat

Derivation
Humanized (from rat)

Type
IgG4 - kappa

Specificity
Tested positive against native human antigen.

Species Reactivity
Human

Applications
WB, ELISA, FC, IP, FuncS, IF, Neut

CAS
1007223-17-7

Generic Name
Olokizumab

Related Disease
Autoimmune diseases

Product Property

Purity
>95.0% as determined by analysis by SDS-PAGE.

Storage
Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

Application Notes
The IL6 antibody has been reported in applications of Neut.
MTT: 2,000 cells/well were seeded in 96-well plates in culture medium (RPMI 1640 supplemented with 10% FBS and penicillin/streptomycin) containing different concentrations of siltuximab. After 96 hours of culture, 10 μL MTT (5 mg/mL in PBS) were added to each well and the plates were incubated for 4 hours. The resulting formazan product was dissolved with acid-isopropanol and the absorbance at a wavelength of 490 nm (A490) was read on a SPECTRAmax Microplate Spectrophotometer.

Target

Alternative Names
Olokizumab;1007223-17-7;CDP6038;IL6;interleukin 6 (interferon, beta 2);IFNB2;interleukin-6;BSF2;HGF;HSF;IL 6;CDF;BSF-2;IFN-beta-2;interferon beta-2;interleukin BSF-2;hybridoma growth factor;CTL differentiation factor;B-cell stimulatory factor 2;B-cell dif

Gene ID
3569

UniProt ID
P05231

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

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There are currently no Customer reviews or questions for TAB-253. Click the button above to contact us or submit your feedback about this product.

Breast cancer biomarkers at key points during disease progression

Great for IL-6 research

This recombinant anti-IL-6 antibody (Olokizumab) is a high-quality reagent. It's a humanized IgG4-kappa derived from a rat antibody, which is an interesting engineering background. I'm using it for functional studies and neutralization assays, both of which are listed as applications. The>95% purity is also excellent.

Works well in FC

I've used this antibody (TAB-253) for flow cytometry (FC) to detect human IL-6, and it works as expected. The immunogen is listed as the recombinant full-length human protein, and it's confirmed to test positive against the native human antigen, giving me confidence in its binding specificity in my assays.

Q&As

What is the host animal and derivation of this Olokizumab antibody?

A: This product, Olokizumab (TAB-253), is a recombinant antibody. Its original host animal was a Rat. It has since been engineered as a Humanized antibody, meaning the antigen-binding regions from the rat antibody were grafted onto a human antibody framework. Specifically, its isotype is IgG4 - kappa. It is expressed in CHO cells and has a purity greater than 95%.
I'm looking for an antibody for neutralization assays. Is TAB-253 validated for this?

A: Yes, this antibody is well-suited for functional studies. The product data for TAB-253 explicitly lists Neutralization (Neut) as a reported application, in addition to others like WB, ELISA, FC, IP, and IF. It is designed to bind to human IL-6 and has been tested positive against the native human antigen, making it a reliable reagent for your neutralization experiments.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-253, RRID: AB_3111899)

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Biosimilar Overview

Please refer to Olokizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Olokizumab.

Related Diseases

Inflammatory Bowel Diseases

Rheumatoid Arthritis

Graft-versus-Host Disease

AGE-RAGE Signaling Pathway in Diabetic Complications

Related Signaling Pathways

TNF Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-253.pdf

MSDS

MSDS - TAB-253.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "Olokizumab"

Afuco™ Anti-IL6 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-253)
This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to IL-6. It is an immunomodulator. It binds to interleukin 6.

DrugMonitor™ Anti-Olokizumab Antibody (VS-1224-YC839)
Olokizumab, a humanized anti-IL-6 antibody, is currently under investigation for its potential use in treating autoimmune disorders such as rheumatoid arthritis. The DrugMonitor™ Anti-Olokizumab Antibody (VS-1224-YC839) is an anti-drug antibody (ADA) against Olokizumab. This drug-based antibody is raised in mice immunized with the Olokizumab. The anti-Olokizumab antibody may be used in ELISA, pharmacokinetics (PK), and pharmacodynamics (PD) analyses, or serves as a reference standard in ADA assays. It also is an excellent tool for therapeutic drug monitoring, allowing to evaluate the drug efficacy and determine the drug concentration of the Olokizumab in samples.

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